In vitro fertilization (IVF) refers to a procedure designed to overcome infertility for tubal disease in women due to undergo in vitro fertilisation. The additional techniques that are routinely used in IVF include ovarian hyperstimulation to generate multiple eggs, ultrasound-guided transvaginal oocyte retrieval directly from the ovaries, co-incubation of eggs and sperm, as well as culture and selection of resultant embryos before embryo transfer into a uterus.‎Medical uses · ‎Complications · ‎Method · ‎Ethics. However, since its introduction in the U.S. in , IVF and other similar techniques have resulted in more than , babies.


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Or you may be advised that you need an egg donor. Egg retrieval Egg retrieval can be done in your doctor's office or a clinic 34 to 36 hours after the final injection and before ovulation.

IVF Treatment

During egg retrieval, you'll be sedated and given pain medication. Transvaginal ultrasound aspiration is the usual retrieval method. An ultrasound probe is inserted into your vagina to identify follicles. Then a thin needle is inserted into an in vitro fertilization technique guide to go through the vagina and into the follicles to retrieve the eggs.

IVF Treatment & Procedures | IVF Australia

If your ovaries aren't accessible through transvaginal ultrasound, an abdominal surgery or laparoscopy — a procedure in which a tiny incision is made near your navel and a slender viewing instrument laparoscope is inserted — may be used to guide the needle.

The eggs are removed from the follicles through a needle connected to a suction device. Multiple eggs can be removed in about 20 minutes. After egg retrieval, you may experience cramping and feelings of fullness or pressure.

Mature eggs are placed in a nutritive liquid culture medium and incubated. Eggs that appear healthy and mature will be mixed with sperm to attempt to create embryos. However, not all eggs may be successfully fertilized.

Sperm retrieval If you're using your partner's sperm, he'll provide a semen sample at your doctor's office or a clinic through masturbation the morning of egg retrieval. Other methods, such as testicular aspiration — the in vitro fertilization technique of a needle or surgical procedure to extract sperm directly from the testicle — are sometimes required.

Donor sperm also can be used.


Sperm are separated from the semen fluid in the lab. Fertilization In vitro fertilization technique can be attempted using two common methods: During insemination, healthy sperm and mature eggs are mixed and incubated overnight. Intracytoplasmic sperm injection ICSI.

In ICSI, a single healthy sperm is injected directly into each mature egg.

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ICSI is often used when semen quality or number is a problem or if fertilization attempts during prior IVF cycles failed. In certain situations, in vitro fertilization technique doctor may recommend other procedures before embryo transfer. About five to six days after fertilization, an embryo "hatches" from its surrounding membrane zona pellucidaallowing it to implant into the lining of the uterus.

If you're an older woman, or if you have had multiple failed IVF attempts, your doctor might recommend assisted hatching — a technique in which a hole is made in the zona pellucida just before transfer to help the embryo hatch and implant. Embryos are allowed to develop in the incubator until they reach a stage where a small sample can be removed and tested for specific genetic diseases or the correct number of chromosomes, typically after five to six days of development.

Embryos that don't contain affected genes or chromosomes can be transferred to your uterus.

In Vitro Fertilization (IVF): Side Effects and Risks

While preimplantation genetic testing can reduce the likelihood that a parent will pass on a genetic problem, it can't eliminate the risk. Prenatal testing may still be in vitro fertilization technique.

Embryo transfer Embryo transfer is done at your doctor's office or a clinic and usually takes place two to six days after egg retrieval. You might be given a mild sedative.

The procedure is usually painless, although you might experience mild cramping.

The doctor or nurse will insert a long, thin, flexible tube in vitro fertilization technique a catheter into your vagina, through your cervix and into your uterus.

A syringe containing one or more embryos suspended in a small amount of fluid is attached to the end of the catheter. Using the syringe, the doctor or nurse places the embryo or embryos into your uterus.